多/单 抗制备
免疫动物（小鼠，兔子），Elisa，WB，IHC
WB
Western Blotting or immunoblotting allows investigators to determine, with a specific primary antibody, the relative amounts of the protein present in different samples.
Briefly,
1) Samples are prepared from tissues or cells that are homogenized in a buffer that protects the protein of interest from degradation;
2) The sample is separated using SDS-PAGE and then transferred to a membrane for detection;
3) The membrane is incubated with a generic protein to bind to any remaining sticky places on the membrane. A primary antibody is then added to the solution which is able to bind to its specific protein;
4) A secondary antibody-enzyme conjugate, which recognizes the primary antibody is added to find locations where the primary antibody bound.
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