Pouring the Gels:

 

Choose a percentage acrylamide based on the molecular weight range of proteins you wish to separate:

% Gel	M.W. Range
7	50 kDa - 500 kDa
10	20 kDa - 300 kDa
12	10 kDa - 200 kDa
15	3 kDa - 100 kDa

 

 

Preparing your Sample:

 

Mix your protein 4:1 with the sample buffer. Heat your sample by boiling for 5-10 minutes .

 

 

Running your gel:

 

Clamp in your gel and fill both buffer chambers with gel running buffer according to the instructions for your specific apparatus.

Pipet your sample into the gel adjusting the volume according to the amount of protein in your sample.

Visualize your proteins using Coomassie Brilliant Blue, Silver stain, or any of the other protein stains.